Prepared Plated Media PPM

SIM (SULFIDE, INDOLE, MOTILITY) MEDIUM

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Catalog Number : 911

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Description

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SIM MEDIUM is a semisolid medium used for the diff erentiation of enteric bacteria through sulfi de production, indole formation and motility. The Sulfur reduction test is useful in diff erentiating enteric organisms, especially Salmonella and Shigella. The Indole test is used for diff erentiating the Enterobacteriaceae. The Motility test is useful for testing a wide variety of organisms. The medium is also useful in the diff erentiation of Klebsiella from Enterobacter and Serratia species. Casein and Meat peptones provide nitrogen, vitamins, minerals and amino acids essential for growth. Casein peptone is rich in tryptophan which is reduced and produces indole. Sodium thiosulfate provides Sulphur and Ferric ammonium citrate is the indicator for H2S production under alkaline conditions. Bacteriological agar is the solidifying agent in a low concentration to enable the motility to be seen.

Inoculate the pure culture by stabbing to a depth of 3/4 of the tube. Incubate at 35 ± 2°C for 18 - 24 hours and read the results. Darkening indicates the production of H2 S. Motility is indicated by a diff use turbidity away from the line of inoculation. Growth only along the inoculation line indicates non-motility. The presence of indole is tested by adding Kovac’s Reagent (Cat. 5205) giving a purple-red coloration to the reagent.

MICROBIOLOGICAL TEST
The following results were obtained in the performance of the medium from type cultures after incubation at a temperature of 35 ± 2°C and observed after 18 - 24 hours.