Prepared Plated Media PPM

THIOGYCOLLATE FLUID MEDIUM

---

Catalog Number : 951

---

Description

---

THIOGLYCOLLATE FLUID MEDIUM is used for detecting microorganisms in sterility tests, according to the formula specifi ed in the US Pharmacopoeia and conforms to formulations detailed in the British and European Pharmacopoeia in the Paragraph 2.6.1 Sterility. Casein peptone provides nitrogen, vitamins, minerals and amino acids essential for growth. Yeast extract is a source of vitamins, particularly of the B-group. Sodium thioglycollate neutralizes the bacteriostatic eff ect of the compounds used as preservatives in pharmaceutical preparations, especially injectables. Sodium thioglycollate and L-Cystine lower the oxidation-reduction potential by removing oxygen to maintain a low pH. Dextrose is the carbohydrate energy source and allows for a rapid and vigorous growth. Sodium chloride supplies essential electrolytes for transport and osmotic balance. Resazurin is an oxidation indicator, turning from pink (aerobic) to colorless (anaerobic conditions). Bacteriological agar delays the dispersion of CO2 and diff usion of O2. With this medium it is not necessary to use a cap of sterile paraffi n oil or incubate in special containers for anaerobes. The anaerobic organisms develop at the bottom of the tube, the microaerophiles in the middle of the medium and the aerobes in the top oxidized layer. Inoculate and incubate at a temperature of 35 ± 2°C. no more than 3 days.

The European Pharmacopoeia recommends in the Paragraph 2.6.1 Sterility the following:For products containing a mercurial preservative that cannot be tested by the membrane-fi ltration method, fl uid thioglycolate medium incubate at 20 - 25°C may be used instead of soya-bean casein digest medium.For growth promotion test of aerobes, anaerobes and fungi, inoculate portions of Fluid thioglycollate medium with a small number, not more than 100 CFU, of Clostridium sporogenes ATCC 11437, Pseudomonas aeruginosa ATCC 9027 and Staphylococcus aureus ATCC 6538. For sterility, incubate portions of the media for 14 days. No growth of microorganisms occurs. When the material in study contains other preservatives, use a suffi cient amount of thioglycollate to dilute the inoculum beyond its bacteriostatic strength level. 

MICROBIOLOGICAL TEST
The following results were obtained from type cultures in the performance of the medium after incubation at a temperature of 35 ± 2°C and observed after 24 hours.