Prepared Plated Media PPM

TRYPTICASEIN SOY AGAR

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Catalog Number : 915

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Description

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TRYPTICASEIN SOY AGAR (TSA) is a general purpose medium very rich in nutrients for general use in microbiological laboratories and for the cultivation and isolation of fastidious or non fastidious microorganisms, or for the maintenance of stock culture. It supports the abundant growth of fastidious organisms such as pneumococci, streptococci, Neisseria, etc. from clinical samples.

Containing two peptones as rich nitrogen sources, obtained by the enzymatic hydrolysis of Casein and Soy proteins, this medium supports the growth of a great variety of microorganisms, including fastidious aerobes and anaerobes. Soy peptone also contains natural sugars which promote bacterial growth. Sodium chloride supplies essential electrolytes for transport and osmotic balance. Bacteriological agar is the solidifying agent.

Some of the microorganisms that grow on this medium are the following: Streptococcus, Neisseria, Brucella, Corynebacteria, Listeria, Pasteurella, Vibrio, Haemophilus vaginalis, Candida, etc. Since it lacks carbohydrates it is very useful in the study of hemolytic reactions and also in the preparation of chocolate agar. If desired, antibiotics can easily be incorporated as well as other supplements or inhibitory agents. Inoculate and incubate at 30 - 35ºC and observe after 18 - 24 hours.The European Pharmacopoeia recommends this medium in the Paragraph 2.6.12 “Microbiological examination of non-sterile products: Microbial enumeration test” for the preparation of test strain. Inoculate and incubate at 30 - 35ºC for 18 - 24 hours for Staphylococcus aureus ATCC 6538, Pseudomonas aeruginosa ATCC 9027 and Bacillus subtilis ATCC 6633. For total aerobic microbial count (growth promotion) of Staphylococcus aureus ATCC 6538, Pseudomonas aeruginosa ATCC 9027 and Bacillus subtilis ATCC 6633, inoculate ≤100 cfu/ml at 30 - 35ºC for ≤ 3 days, of and for Candida albicans ATCC 10231 and Aspergillus brasiliensis ATCC 16404 inoculate ≤100 cfu/ml at 30 - 35 ºC ≤ 5 days. For total aerobic microbial count (Suitability of counting method in the presence of the product) inoculate ≤100 cfu/ml of Staphylococcus aureus, Pseudomonas aeruginosa and Bacillus subtilis at 30 - 35ºC for ≤ 3 days, and for Candida albicans ATCC 10231 and Aspergillus brasiliensis ATCC 16404 inoculate ≤100 cfu/ml at 30 - 35 ºC ≤ 5 days.

MICROBIOLOGICAL TEST
The following results were obtained in the performance of the medium, with the blood added, from type cultures after incubation at a temperature of 30 - 5ºC and observed after 18 - 24 hours.